Archimed X4 Real-Time qPCR System
Archimed X4 Real-Time qPCR System
Features
1. Innovative Optical Design
(1) High sensitivity
(2) Less cross-talk
(3) Fast scan
(4) No edge effects
(5) Maintenance-free
2. Outstanding Thermal Cycler
(1) Compatible with 0.1ml or 0.2ml Low Profile 96-well plate, 8-strip tube, single tube(transparent, frosted, and milky white are applicable)
(2) Precise temperature control of± 0.2 ℃ across the entire sample block
(3) Innovative thermal block with max ramp rates of up to 3.6 ℃/s
(4) Gradient function over 12 columns with a 36 ℃ spread
3. Reliable Results with High-quality Data
(1) Excellent reproducibility and 10-log dynamic range
(2) Precise quantification with 1.33-fold discrimination
(3) Broad linear dynamic range ensuring accurate quantification
4. Intelligent Analysis for Multiple Applications
(1) Accommodates user needs and different types of experiments with intuitive navigation and customizable settings.
(2) Built-in data analysis modules with automatic baseline subtraction and threshold calculation for determining Ct values or possible standard curves and PCR efficiencies
(3) Absolute quantification or relative quantification applicable
(4) Includes analysis methods for probe-based allelic discrimniation and the use of a positive/negative analysis via the end-point detection of samples
Specifications
Thermal Cycler | |
Block capacity | 96 |
Sample volume | 10-50μl |
Heating/cooling method | Peltier |
Max ramp rate | 3.6℃/sec |
Temperature setting range | 4-100℃ |
Heated lid | Electronic automatic lid |
Temperature accuracy | ±0.2℃ |
Temperature uniformity | ±0.2℃ |
Gradient zone | 12 columns |
Gradient range | 1-36℃ |
Optical Detection | |
Excitation source | Long-life, high-performance LEDs |
Detector | Highly sensitive MPPC with Fresnel lens |
Scanning principle | Time-resolved scanning technology |
Detector position | Top of the block |
Excitation/detection range | 455-650nm/510-715nm |
Fluorescence channel | 4 channels (Archimed X4) 6 channels (Archimed X6) |
Detection sensitivity | 1 copy of the target sequence |
System sensitivity | Detect differences as small as 1.33-fold in target quantities in singleplex reactions |
Dynamic range | 10 orders of magnitude |
Dye compatibility | FAM/SYBR Green, VIC/JOE/HEX/TET, NED/TAMRA/Cy3, JUN, ROX/Texas Red, Mustang Purple, Cy5/LIZ |
Data Analysis Modes | |
Absolute quantification Relative quantification Endpoint qualitative analysis Melt curve analysis Protein Stability Screening Genotyping |
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Data Export | |
Customizable reports containing run settings, data graphs, and spreadsheets can be directly exported or saved as Excel, txt, PDFs |