Based on the conventional fluorescence PCR instrument, Sansure has independently developed and upgraded "Multiplex fluorescent PCR-amplification technology" from the aspects of amplification reaction buffer system and probe labeling technology, etc., This technology creatively combined with the detection technology of TaqMan probe and melting curve. It also overcomes the limitation of single channel detection of one target, realize the detection and analysis of at least two targeted targets in the monochromatic fluorescent channel simultaneously, detect at least eight targets in one reaction tube at the same time, and realize the rapid and accurate identification and diagnosis of multiple pathogens.